The Effection of L-Pyroglutamic Acid
L-Pyroglutamic acid is also called 5-Oxopyrrolidine-2-carboxylic acid or L-5-Pyrrolidone-2-carboxylic acid. It comprises constitute in many proteins including bacteriorhodopsin. It appears as a white to light yellow crystal powder.
Pyroglutamic acid (also known as 5-oxoproline, pidolic acid, or pyroglutamate for its basic form) is an uncommon amino game breaker differential inward which the free amino group of glutamic acid cyclizes to form a lactam. It is an unusual amino acid derivative in which the free amino group of glutamic acid cyclizes to form a lactam. L-Pyroglutamic Acid is found in various proteins including bacteriorhodopsin.
L-Pyroglutamic Acid is believed to be associated with activity of the key neurotransmitter, acetylcholine, and with the production of natural GABA and glycine, two other important neurotransmitters. It is an amino acid found in fruits and vegetables. N-terminal glutamine residues can spontaneously cyclize to become pyroglutamate.
Regarding the enzyme activities, PGA significantly decreased NADH: cytochrome c oxireductase (complex I plus CoQ plus complex III) by 40% at concentrations of 0.5-3.0 mMand cytochrome c oxidase activity by 22-30% at the concentration of3.0 mM, without affecting the activenesses of succinate dehydrogenase, succinate:DCPIP oxireductase (complex II).
This is one of several forms of blocked N-terminals which present a problem for N-terminal sequencing using Edman chemistry, which requires a free primary amino group not introduce successful pyroglutamic dose. The enzyme pyroglutamate aminopeptidase can restore a free N-terminus by cleaving off the pyroglutamate residual.