李興旺 博士 教授 博士生導師

教育經歷（從大學本科開始）:

1999.9-2003.7 內蒙古農業大學 生物工程學院 生物技術專業 理學學士學位

2003.9-2011.3 華中農業大學 生命科學技術學院 生物化學與分子生物學專業 理學博士學位

科研與學術工作經歷:

2016.8-現在 華中農業大學 生命科學與技術學院 作物遺傳與改良國家重點實驗室 教授

2013.4-2016.7 The Jackson Laboratory for Genomic Medicine 博士后

2011.4-2013.3華中農業大學 作物遺傳與改良國家重點實驗室 Research Associate

研究方向:

真核生物的線性基因組在細胞核內組織成一定的三維空間結構，在基因轉錄調控和個體發育中發揮重要作用。我們課題組圍繞水稻雜種優勢形成和晝夜節律過程中的基因組三維結構參與基因轉錄調控的機制這一基本科學問題，利用基因組學、表觀遺傳學、生物信息學、分子遺傳學和生物化學等多種研究手段，研究水稻基因組中線性距離較遠的各種DNA順式調控元件（如啟動子、增強子和絕緣子等）或基因之間的遠程相互作用；揭示參與順式調控元件三維互作的蛋白質復合體及非編碼RNA的組成及功能。從三維基因組組角度理解基因的轉錄調控及其控制水稻重要農藝形狀的分子機理。課題組的遠期目標是運用CRISPR及其多種衍生技術，人工改造水稻的表觀遺傳修飾和順式調控元件的遠程相互作用，改良水稻品種。

本課題組將培養同時具備多種實驗設計和操作技能及高通量組學數據處理和分析能力的新一代科學家。歡迎對課題組研究方向感興趣的研究生、博士后和科研助理加入。

代表性研究成果:

1. Lun Zhao*, Shuangqi Wang*, Zhilin Cao*, Weizhi Ouyang, Qing Zhang, Liang Xie, Ruiqin Zheng, Minrong Guo, Meng Ma, Zhe Hu, Wing-Kin Sung, Qifa Zhang, Guoliang Li^#, Xingwang Li^# (2019) Chromatin loops associated with active genes and heterochromatin shape rice genome architecture for transcriptional regulation. Nature Communications. 10: 3640.

2. Yong Peng*, Dan Xiong*, Lun Zhao, Weizhi Ouyang, Shuangqi Wang, Jun Sun, Qing Zhang, Pengpeng Guan, Liang Xie, Wenqiang Li, Guoliang Li^#, Jianbing Yan^#, Xingwang Li^# (2019) Chromatin Interaction Maps Reveal Genetic Regulation for Quantitative Traits in Maize. Nature Communications. 10: 2632.

3. Xingwang Li*, Oscar Luo*, Ping Wang, Meizhen Zheng, Danjuan Wang, Emaly Piecuch, Jacqueline Jufen Zhu, Simon Tian, Zhonghui Tang, Guoliang Li, Yijun Ruan. (2017) Long-read ChIA-PET for base-pair resolution mapping of haplotype-specific chromatin interactions. Nature Protocol. 12: 899–915.(*Co-first author)

4. Zhonghui Tang*, Oscar Luo*, Xingwang Li*, Meizhen Zheng, Jacqueline Jufen Zhu, Przemyslaw Szalaj, Pawel Trzaskoma, Adriana Magalska, Jakub Wlodarczyk, Blazej Ruszczycki, Paul Michalski, Emaly Piecuch, Ping Wang, Danjuan Wang, Simon Zhongyuan Tian, Xiaoan Ruan, May Penrad-Mobayed, Laurent M. Sachs, Chia-Lin Wei, Edison T. Liu, Grzegorz M. Wilczynski, Dariusz Plewczynski, Guoliang Li, Yijun Ruan. (2015) CTCF-mediated 3D genome architecture reveals a chromatin topology for transcription regulation. Cell. 163: 1611–1627. (*Co-first author)

5. Xingwang Li and Changyin Wu. (2014) Rice Meiotic Chromosome Spread Preparation of Pollen Mother Cells. Bio-protocol 4(14): e1189.

6. Xingwang Li, Yuxiao Chang, Xiaodong Xin, Chunmei Zhu, Xianghua Li, James D Higgins, Changyin Wu. (2013) Replication Protein A2c Coupled with Replication Protein A1c Regulates Crossover Formation during Meiosis in Rice. Plant Cell. 25: 3885–3899.

7. Xingwang Li, Xingqiang Gao, Yi Wei, Li Deng, Yidan Ouyang, Guoxing Chen, Xianghua Li, Qifa Zhang, Changyin Wu. (2011) Rice Apoptosis inhibitor 5 coupling with two DEAD-box ATP-dependent RNA helicases regulates the PCD process of tapetum degeneration. Plant Cell. 23:1416–1434.

8. Laura Vian, Aleksandra Pekowska, Suhas Rao, Kyong-Rim Kieffer-Kwon, Seolkyoung Jung, Laura Baranello, Su-Chen Huang, Laila El Khattabi, Marei Dose, Nathanael Pruett, Adrian Sanborn, Andres Canela, Yaako Maman, Anna Oksanen, Wolfgang Resch, Xingwang Li, Byoungkoo Lee, Alexander Kovalchuk, Zhonghui Tang, Steevenson Nelson, Pierro Di, Cheng Michele, Machol Ryan, Ido St Hilaire, Glenn Brian, Neva Durand, Muhammad Shamim, Elena Stamenova, Jose Onuchic, Yijun Ruan, Andre Nussenzweig, David Levens, Erez Lieberman Aiden, Rafael Casellas. (2018) The Energetics and Physiological Impact of Cohesin Extrusion. Cell.173: 1165–1178.

9. Peng Li, Suman Mitra, Rosanne Spolski, Jangsuk Oh, Wei Liao, Zhonghui Tang, Fei Mo, Xingwang Li, Erin West, Daniel Gromer, Jian-Xin Lin, Chengyu Liu, Yijun Ruan, Warren Leonard. (2017) STAT5-mediated chromatin interactions in superenhancers activate IL-2 highly inducible genes: Functional dissection of the Il2ra gene locus. Proc Natl Acad Sci U S A. 114:12111–12119.

10. Isis Ricano-Ponce, Daria Zhernakova, Patrick Deelen, Oscar Luo, Xingwang Li, Aaron Isaacs, Juha Karjalainen, Jennifer Di Tommaso, Zuzanna Agnieszka Borek, Maria Zorro, Javier Gutierrez-Achury, Andre Uitterlinden, Albert Hofman, Joyce van Meurs, Mihai Netea, Iris Jonkers, Sebo Withoff, Cornelia van Duijn, Yang Li, Yijun Ruan, Lude Franke, Cisca Wijmenga, Vinod Kumar. (2016) Refined mapping of autoimmune disease associated genetic variants with gene expression suggests an important role for non-coding RNAs. J Autoimmun. 68:62–74.

11. Przemyslaw Szalaj, Zhonghui Tang, Paul Michalski, Michal Pietal, Oscar Luo, Michal Sadowski, Xingwang Li, Kamen Radew, Yijun Ruan, Dariusz Plewczynski. (2016) An integrated 3-Dimensional Genome Modeling Engine for data-driven simulation of spatial genome organization. Genome Res. 26: 1697–1709.

12. Xinqiang Gao, Zhihui Chen, Jian Zhang, Xingwang Li, Guoxing Chen, Xianghua Li, Changyin Wu. (2012) OsLIS-L1 encoding a lissencephaly type-1-like protein with WD40 repeats is required for plant height and male gametophyte formation in rice. Planta. 353: 713–727.

13. Yuxiao Chang, Liang Gong, Wenya Yuan, Xingwang Li, Guoxing Chen, Xianghua Li, Qifa Zhang, Changyin Wu. (2009) Replication protein A (RPA1a) is required for meiotic and somatic DNA repair but is dispensable for DNA replication and homologous recombination in rice. Plant Physiol. 15: 2162–2173.

14. Wenya Yuan, Xingwang Li, Yuxiao Chang, Ruoyu Wen, Guoxing Chen, Qifa Zhang, Changyin Wu. (2009) Mutation of the rice gene PAIR3 results in lack of bivalent formation in meiosis. Plant J. 59: 303–315.

15. Jian Zhang, Dong Guo, Yuxiao Chang, Changjun You, Xingwang Li, Xiaoxia Dai, Qijun Weng, Guoxing Chen, Qifa Zhang, Changyin Wu. (2007) Non-random distribution of T-DNA insertions at various levels of the genome hierarchy as revealed by analyzing 13804 T-DNA flanking sequences from an enhancer-trap mutant library. Plant J. 49: 947–959.

授權專利

其他成果

獲得獎勵

主講課程

生信信息學；生命科學導論MOOC；分子克隆技術

研究生招生專業

基因組學；生物化學與分子生物學

聯系方式

實驗室電話：

電子郵箱：xingwangli@mail.hzau.edu.cn

辦公地點：作物遺傳改良國家重點實驗室（第二綜合樓）B613-1

實驗室介紹及網址：